Comparative Evaluation Of Cytotoxicity Of Carica Papaya Leaf And Seed Extract - A Cell Line Study

Abstract

Introduction Carica papaya is recognized for its ample therapeutic potential. A range of papaya components including seeds, leaves, fruit, and latex are globally employed for therapeutic purposes, serving to promote health and mitigate various ailments. The literature has also documented the noteworthy anti-inflammatory and antioxidant attributes of Carica papaya. The aim of the present study is to evaluate and compare the cytotoxicity of papaya leaf and seed extract.

Materials and Method Ethanolic extract of various concentrations, ranging from 0.2-1 mg/ml of the papaya leaf and the seed extract were tested in mouse fibroblast, McCoy cells using the MTT colourimetric assay after 48 hours of incubation.

Results C. Papaya leaves and seed extracts had a non-significant effect on cell growth in McCoy cells in a dose-dependent state after 48 hours of treatment. The viability of cells with Papaya leaf extract was significantly higher than that of papaya seed extract (p<0.05). The result demonstrated that the treatment of C. papaya extracts in an increasing concentration shows a slight cytotoxic in higher concentration in McCoy, mouse fibroblast cells

Conclusion Carica papaya leaf and seed extract had a non-significant effect on cell growth in McCoy cells in a dose-dependent state after 48 hours of treatment. The papaya leaf extract was significantly better than the seed extract on the basis of the percentage of viable cells in the culture. This study demonstrated the high safety of the papaya extracts to be used as drugs in therapeutic practices.