Comparative Evaluation Of Antibacterial Efficacy Of Ocimum Tenuiflorum And Syzygium Aromaticum Formulations As An Intracanal Medicament Compared To With Calcium Hydroxide On E. Faecalis

Abstract

Background:Intracanal medicament is a fundamental part of endodontic procedures that must have an antibacterial impact on the infected root canal bacteria for endodontic procedures to be effective. Calcium hydroxide is the current "gold standard" intracanal medication. The process of seeking for an effective and perfect intracanal medication is a constant and challenging effort. In recent years, there has been increasing interest in herbal products due to their antibacterial, antiseptic, and analgesic characteristics.

Aim: To assess the antibacterial activity of herbal extracts of tulsi and clove with calcium hydroxide as a control, in vitro assessment as an intracanal medicament on Enterococcus faecalis.

Materials And Method: This is an in vitro study using E. Faecalis, which is typically detected in infected root canals. E. faecalis was inoculated onto a Mueller Hinton agar plate. Wells with diameters of 6-8mm were punched and the herbal extract mixtures pipetted into these agar wells. After 48 hours of incubation, the diameter of the inhibition zones around all of the wells was measured manually. The inhibition zones of tulsi and clove combinations were then compared to the inhibition zone of calcium hydroxide.

Results: The herbal based intracanal medicament showed antimicrobial effect against  test microbes, where the mean value of inhibition zone size (mm) was increased by increasing the concentration.When compared to tulsi and its mixtures, calcium hydroxide herbal formulations demonstrated superior antibacterial activity.

Conclusion :The herbal based intracanal medicament seems to be more effective against E.Faecalis compared to that of calcium hydroxide.Herbal based intracanal medicament performed better antibacterial efficacy as compared to conventional calcium hydroxide.