Comparative Toxicity Study of EverX Posterior and SDR Flow+ in Zebrafish Embryo Model

Abstract

Objective: This study aimed to assess the potentially toxic effects of EverX Posterior and SDR Flow+ dental materials on zebrafish embryos, using various endpoints such as mortality rate, hatching rate, heart rate, and morphological malformations.

Materials and Methods: Zebrafish embryos were exposed to EverX Posterior and SDR Flow+ dental materials from 4 hours post-fertilization (hpf) to 96 hpf. Parameters including survival, hatching rate, heart rate, and morphological malformations were evaluated. Statistical analysis was performed using one-way ANOVA and Tukey's post hoc test.

Results: EverX Posterior did not cause mortality in zebrafish embryos, while the mortality rate was higher in the SDR Flow+ group. The hatching rate was similar between the EverX Posterior and control groups, but significantly decreased in the SDR Flow+ group. The heart rate of zebrafish embryos exposed to EverX Posterior was not significantly different from the control group, whereas SDR Flow+ resulted in a significant decrease in heart rate. Morphological analysis revealed normal architecture in the EverX Posterior group, while SDR Flow+ treated embryos exhibited malformations and higher mortality.

Conclusion: The study findings suggest that SDR Flow+ had a slightly toxic effect on zebrafish embryos compared to EverX Posterior. Further investigations are warranted to better understand the underlying mechanisms and refine dental materials for enhanced safety and biocompatibility.