Evaluating the toxicity and antioxidant activity of Oligopeptide with remineralizing potential on Zebrafish larvae

Abstract

Aim: The aim of the study is to assess the ability of self-assembling peptide (p11-4) with varying concentrations based on diffusion and remineralization potential on early carious lesions.

Objectives: To evaluate the antioxidant property of p 11 4 self-assembling peptide and to determine the toxicity level of the p 11 4 peptide in zebrafish larvae and embryo .

Materials and Methods: Adult zebrafish (4 months old) were purchased from a local aquarist. The male and female fishes were separated, maintained in our facility under the following condition in a 10 L glass tank: 28.5˚C, with a 14/10 h light/dark cycle. The fishes were acclimatised for 1 month, later the fishes were utilised for breeding, and embryos were collected and used for the following experiments. The collected embryos were further analysed under microscope.

Results: The survival rate of zebrafish larvae was investigated after being exposed with different groups of P114 peptide for 24hrs. (p > 0.001) indicates the significant difference between the control (untreated larvae) and treated group.

Conclusion: According to this study, it indicates that P114 peptide has antioxidant activity. It helps to maintain the antioxidant enzyme level during the oxidative stressed condition. The SOD and CAT level are upregulated during P114 peptide.