Molecular mechanism of formulations mediated (amla, neem) silver nanoparticles in e. Faecalis

Abstract

Aim : The aim of the study is to evaluate the molecular mechanism of  herbal formulations mediated silver nanoparticles against different organisms .

Materials and methods : Azadirachta indica and Amla leaves were dried and powdered , which were made into herbal formulation . Silver nitrate (1 millimolar ) was dissolved in 80 ml of distilled water , to that 20ml of filtered Amla , Neem plant extracts were added and kept in an orbital shaker for 2 days . To determine the antibacterial activity of silver nanoparticles Agar well diffusion method was done . Bacterial suspensions were dispersed with various organisms on the surface of plates containing Muller -Hinton agar . Readings were taken using U.V spectrophotometer at ( 250 -750 nm ) and Centrifugation done ( 8000 rpm for 10 mins ) Pellets were collected .Statistical analysis done using One way ANOVA and post Hoc test . P value was analysed.

Results :After the synthesis of silver nanoparticles color change was observed , the particles collected were then characterized and the peak value was seen at 425nm using UV- Spectroscopy . As the concentration of silver nanoparticles increases the zone of inhibition also increases in size.

Conclusion: Dental materials' antibacterial capabilities might be improved by adding silver nanoparticles to them. The Amla and Neem extracts used to synthesise the silver nanoparticles have also demonstrated anti-inflammatory properties. As a non-toxic substitute for traditional antibacterial treatments, silver nanoparticles may be utilised.